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Differential viral haemorrhagic septicaemia virus genotype IV b infection in fin fibroblast and epithelial cell lines from walleye, S ander vitreus ( M itchill), at cold temperatures
Ist Teil von
Journal of fish diseases, 2016-02, Vol.39 (2), p.175-188
Erscheinungsjahr
2016
Quelle
Wiley Online Library
Beschreibungen/Notizen
Abstract
A cell line,
WE
‐cfin11e, with an epithelial‐like morphology was developed from a caudal fin of walleye,
S
ander vitreus
(
M
itchill), characterized as distinct from the established walleye caudal fin fibroblast‐like cell line,
WE
‐cfin11f, and compared with
WE
‐cfin11f for susceptibility to
VHSV IV
b. Immunocytochemistry and confocal microscopy were used to localize the intermediate filament protein, vimentin, the tight junction protein, zonula occludens‐1 (
ZO
‐1), the extracellular matrix protein, collagen
I
, and the viral protein,
G
. Although both cell lines contained vimentin, only
WE
‐cfin11e stained for
ZO
‐1 and only
WE
‐cfin11f stained for collagen
I
. Ascorbic acid increased the accumulation of collagen
I
and caused the appearance of collagen fibres only in
WE
‐cfin11f cultures. At 14 °C, both cell lines produced
VHSV IV
b, but the infection developed more rapidly in
WE
‐cfin11f. At 4 °C, both cell lines became infected with
VHSV IV
b as judged by the expression of viral proteins,
N
and
G
, but only
WE
‐cfin11f produced virus. The results suggest that cold temperatures can modulate viral tropism.
Sprache
Englisch
Identifikatoren
ISSN: 0140-7775
eISSN: 1365-2761
DOI: 10.1111/jfd.12345
Titel-ID: cdi_crossref_primary_10_1111_jfd_12345
Format
–
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