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When the frogs are placed in ice‐water for one hour and then bled, plasma potassium in most cases is increasing. Addition of adrenaline and noradrenaline make the blood cells absorb potassium.
Adrenaline has an effect down to 0.01 μg/ml, noradrenaline is from 10 to 100 times less efficient.
Hppertonic plasma plus adrenaline or noradrenaline produces extremely low potassium values (below 0.5 mM/1).
When pigeon blood is rocked at 43° for one and a half hour the plasma concentration is rising. The pigeon blood cells are now very sensitive to adrenaline and noradrenaline (noradrenaline 2–3 times more efficient).
Down to 0.001 μg/ml or perhaps even 0.0001 μg/ml of noradrenaline has an effect.
It is of importance that all the samples are dealt with in exactly the same way (dilution. shaking etc.)