Details

Autor(en) / Beteiligte

• Sponder, Gerhard
• Svidova, Sona
• Schindl, Rainer
• Wieser, Stefan
• Schweyen, Rudolf J.
• Romanin, Christoph
• Froschauer, Elisabeth M.
• Weghuber, Julian

Titel

Lpe10p modulates the activity of the Mrs2p‐based yeast mitochondrial Mg 2+ channel

Ist Teil von

• The FEBS journal, 2010-09, Vol.277 (17), p.3514-3525

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Saccharomyces cerevisiae Lpe10p is a homologue of the Mg 2+ ‐channel‐forming protein Mrs2p in the inner mitochondrial membrane. Deletion of MRS2 , LPE10 or both results in a petite phenotype, which exhibits a respiratory growth defect on nonfermentable carbon sources. Only coexpression of MRS2 and LPE10 leads to full complementation of the mrs2 Δ/ lpe10 Δ double disruption, indicating that these two proteins cannot substitute for each other. Here, we show that deletion of LPE10 results in a loss of rapid Mg 2+ influx into mitochondria, as has been reported for MRS2 deletion. Additionally, we found a considerable loss of the mitochondrial membrane potential (ΔΨ) in the absence of Lpe10p, which was not detected in mrs2 Δ cells. Addition of the K + /H + ‐exchanger nigericin, which artificially increases ΔΨ, led to restoration of Mg 2+ influx into mitochondria in lpe10 Δ cells, but not in mrs2 Δ/ lpe10 Δ cells. Mutational analysis of Lpe10p and domain swaps between Mrs2p and Lpe10p suggested that the maintenance of ΔΨ and that of Mg 2+ influx are functionally separated. Cross‐linking and Blue native PAGE experiments indicated interaction of Lpe10p with the Mrs2p‐containing channel complex. Using the patch clamp technique, we showed that Lpe10p was not able to mediate high‐capacity Mg 2+ influx into mitochondrial inner membrane vesicles without the presence of Mrs2p. Instead, coexpression of Lpe10p and Mrs2p yielded a unique, reduced conductance in comparison to that of Mrs2p channels. In summary, the data presented show that the interplay of Lpe10p and Mrs2p is of central significance for the transport of Mg 2+ into mitochondria of S. cerevisiae . Structured digital abstract MINT‐7905005 : LPE10 (uniprotkb: Q02783 ) physically interacts ( MI:0915 ) with MRS2 (uniprotkb: Q01926 ) by anti tag coimmunoprecipitation ( MI:0007 ) MINT‐7905028 : LPE10 (uniprotkb: Q02783 ) and LPE10 (uniprotkb: Q02783 ) covalently bind ( MI:0195 ) by cross‐linking study ( MI:0030 ) MINT‐7905072 : LPE10 (uniprotkb: Q02783 ) and MRS2 (uniprotkb: Q01926 ) covalently bind ( MI:0195 ) by cross‐linking study ( MI:0030 )