Details

Autor(en) / Beteiligte

• Oishi, Kazuhiko
• Mita, Mitsuo
• Ono, Teruaki
• Hashimoto, Takao
• Uchida, Masaatsu K.

Titel

Protein kinase C‐independent sensitization of contractile proteins to Ca 2+ in α‐toxin‐permeabilized smooth muscle cells from the guinea‐pig stomach

Ist Teil von

• British journal of pharmacology, 1992-12, Vol.107 (4), p.908-909

Erscheinungsjahr

1992

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Involvement of protein kinase C in receptor‐operated Ca 2+ sensitization of cell shortening was investigated by use of α‐toxin‐permeabilized smooth muscle cells from the fundus of the guinea‐pig. Most of the isolated cells responded to 0.6 μ m Ca 2+ with a maximal shortening to approximately 65% of the resting cell length. Addition of acetylcholine (ACh) at a maximal concentration (10 μ m ) resulted in a marked decrease in the concentration of Ca 2+ required to trigger a threshold response from 0.6 μ m to 0.2 μ m . The augmentation of Ca 2+ sensitivity by ACh was not inhibited by specific protein kinase C inhibitors, calphostin C and K‐252b at a concentration of 1 μ m . These findings suggest that protein kinase C is not involved in the muscarinic receptor‐operated augmentation of Ca 2+ sensitivity.