Details

Autor(en) / Beteiligte

• Serena, T.
• Li, V. W.
• Li, W. W.

Titel

063
The Human Forearm Biopsy Model for Acute Wound Healing

Ist Teil von

• Wound repair and regeneration, 2004-04, Vol.12 (2), p.A18-A18

Ort / Verlag

Oxford, UK, Malden, USA: Blackwell Science Ltd/Inc

Erscheinungsjahr

2004

Quelle

Wiley Online Library

Beschreibungen/Notizen

• Background: The study of wound biology and effects of advanced modalities would benefit from a reliable human model for normal healing. Eaglstein et al. previously suggested that the forearm biopsy may be useful in acute wound healing studies (J Am Acad Dermatol 45:857, 2001). The Wound Healing Cooperative Group (WHCG) here validates this model in healthy individuals treated with topical growth factor therapy. Methods: In our randomized, double‐blinded study design, 20 normal healthy volunteers underwent four 6 mm biopsies of the flexor surface of both forearms. Biopsy sites were randomly assigned to a control arm (daily bacitracin) or to one of three treatment arms: i) rhPDGF‐BB 0.01% gel (Q.D.), ii) rhPDGF‐BB (Q.O.D.), iii) rhPDGF‐BB (Q.D. × 7 days followed by bacitracin alone daily). The wounds were examined, measured and photographed daily until complete healing was achieved. Adverse events were monitored. Rates of healing and time‐to‐complete closure were measured. Repeat biopsies of a subset of healing wounds were performed for gene microarray analysis. Results: The forearm biopsy model allowed direct quantitative and qualitative comparisons of acute wound healing outcomes achieved by rhPDGF‐BB regimens versus standard care alone. There were no infectious complications. Subject compliance was excellent with < 3% of visits missed. Conclusion: The forearm biopsy model has several advantages in the study of acute wounds: it allows for comparison of topical agents in a controlled fashion; studies can be conducted easily with good patient compliance; and the forearm allows for repeat imaging and tissue harvesting for gene profiling during healing. Acknowledgements: Funding from The Angiogenesis Foundation.