Details

Autor(en) / Beteiligte

• Brenner, Sibylle A.
• Zacheja, Steffi
• Schäffer, Michael
• Feilhauer, Katharina
• Bischoff, Stephan C.
• Lorentz, Axel

Titel

Soluble CD 14 is essential for lipopolysaccharide‐dependent activation of human intestinal mast cells from macroscopically normal as well as C rohn's disease tissue

Ist Teil von

• Immunology, 2014-10, Vol.143 (2), p.174-183

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Summary Mast cells are now considered sentinels in immunity. Given their location underneath the gastrointestinal barrier, mast cells are entrusted with the task of tolerating commensal microorganisms and eliminating potential pathogens in the gut microbiota. The aim of our study was to analyse the responsiveness of mast cells isolated from macroscopically normal and Crohn's disease‐affected intestine to lipopolysaccharide ( LPS ). To determine the LPS ‐mediated signalling, human intestinal mast cells were treated with LPS alone or in combination with soluble CD 14 due to their lack of surface CD14 expression. LPS alone failed to stimulate cytokine expression in human intestinal mast cells from both macroscopically normal and Crohn's disease tissue. Upon administration of LPS and soluble CD 14, there was a dose‐ and time‐dependent induction of cytokine and chemokine expression. Moreover, CXCL 8 and interleukin‐1 β protein expression was induced in response to activation with LPS plus soluble CD 14. Expression of cytokines and chemokines was at similar levels in mast cells from macroscopically normal and Crohn's disease‐affected intestine after LPS /soluble CD 14 treatment. In conclusion, human intestinal mast cells appear to tolerate LPS per se. The LPS ‐mediated activation in mast cells may be provoked by soluble CD 14 distributed by other LPS ‐triggered cells at the gastrointestinal barrier.