Details

Autor(en) / Beteiligte

• Riedel, Franziska
• Aparicio-Soto, Marina
• Curato, Caterina
• Münch, Lucas
• Abbas, Amro
• Thierse, Hermann-Josef
• Peitsch, Wiebke K
• Luch, Andreas
• Siewert, Katherina

Titel

Unique and common TCR repertoire features of Ni 2+ -, Co 2+ -, and Pd 2+ -specific human CD154 + CD4+ T cells

Ist Teil von

• Allergy (Copenhagen), 2023-01, Vol.78 (1), p.270-282

Ort / Verlag

Denmark

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Apart from Ni , Co , and Pd ions commonly trigger T cell-mediated allergic contact dermatitis. However, in vitro frequencies of metal-specific T cells and the mechanisms of antigen recognition remain unclear. Here, we utilized a CD154 upregulation assay to quantify Ni -, Co -, and Pd -specific CD4+ T cells in peripheral blood mononuclear cells (PBMC). Involved αβ T cell receptor (TCR) repertoires were analyzed by high-throughput sequencing. Peripheral blood mononuclear cells incubation with NiSO , CoCl , and PdCl increased frequencies of CD154 + CD4+ memory T cells that peaked at ~400 μM. Activation was TCR-mediated as shown by the metal-specific restimulation of T cell clones. Most abundant were Pd -specific T cells (mean 3.5%, n = 19), followed by Co - and Ni -specific cells (0.6%, n = 18 and 0.3%, n = 20) in both allergic and non-allergic individuals. A strong overrepresentation of the gene segment TRAV9-2 was unique for Ni -specific TCR (28% of TCR) while Co and Pd -specific TCR favorably expressed TRAV2 (8%) and the TRBV4 gene segment family (21%), respectively. As a second, independent mechanism of metal ion recognition, all analyzed metal-specific TCR showed a common overrepresentation of a histidine in the complementarity determining region 3 (CDR3; 15% of α-chains, 34% of β-chains). The positions of the CDR3 histidine among metal-specific TCR mirrored those in random repertoires and were conserved among cross-reactive clonotypes. Induced CD154 expression allows a fast and comprehensive detection of Ni -, Co -, and Pd -specific CD4+ T cells. Distinct TCR repertoire features underlie the frequent activation and cross-reactivity of human metal-specific T cells.