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Free amino acid profile during ripening of ewe's milk cheese from the C roatian island K rk
Ist Teil von
International journal of dairy technology, 2013-08, Vol.66 (3), p.390-395
Erscheinungsjahr
2013
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
The aim of this study was to determine the content of free amino acids (
FAA
) and their ratio in ewe's milk cheese from the island
K
rk during its ripening.
FAA
content was determined by reversed phase
HPLC
(
RP
‐
HPLC
) of cheese aqueous/ethanol extracts after
FAA
were transformed into their 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate derivatives. Their concentration increased during ripening, reaching the value of 5% in cheese dry matter. The dominant
FAA
were glutamic acid>leucine>valine>aspartic acid>phenylalanine>serine>proline, and higher content of nonessential vs essential
FAA
was revealed.
K
rk cheese has, in relation to other cheeses, higher values for glutamic acid/leucine, glutamic acid/phenylalanine, glutamic acid/proline and smaller values for leucine/aspartic acid, valine/aspartic acid, phenylalanine/aspartic acid ratios, while other ratios are comparable to those of other hard ovine cheeses.