Plant physiology (Bethesda), 2020-02, Vol.182 (2), p.892-907
2020
Details
- Autor(en) / Beteiligte
- Titel
- Exogenous Auxin Induces Transverse Microtubule Arrays Through TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX Receptors
- Ist Teil von
- Plant physiology (Bethesda), 2020-02, Vol.182 (2), p.892-907
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Auxin plays a central role in controlling plant cell growth and morphogenesis. Application of auxin to light-grown seedlings elicits both axial growth and transverse patterning of the cortical microtubule cytoskeleton in hypocotyl cells. Microtubules respond to exogenous auxin within 5 min, although repatterning of the array does not initiate until 30 min after application and is complete by 2 h. To examine the requirements for auxin-induced microtubule array patterning, we used an Arabidopsis ( ) double ( ) receptor mutant, , that responds to conventional auxin (indole-3-acetic acid) but has a strongly diminished response to the auxin analog, picloram. We show that 5 µm picloram induces immediate changes to microtubule density and later transverse microtubule patterning in wild-type plants, but does not cause microtubule array reorganization in the mutant. Additionally, a dominant mutant ( ) for the auxin coreceptor AUXIN RESPONSIVE2 (AXR2) was strongly suppressed for auxin-induced microtubule array reorganization, providing additional evidence that auxin functions through a transcriptional pathway for transverse patterning. We observed that brassinosteroid application mimicked the auxin response, showing both early and late microtubule array effects, and induced transverse patterning in the mutant. Application of auxin to the brassinosteroid synthesis mutant, , induced transverse array patterning but did not produce significant axial growth. Thus, exogenous auxin induces transverse microtubule patterning through the TRANSPORT INHIBITOR 1/AUXIN F-BOX (TIR1/AFB) transcriptional pathway and can act independently of brassinosteroids.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.19.00928Titel-ID: cdi_crossref_primary_10_1104_pp_19_00928
- Format
- –
- Schlagworte
- Arabidopsis - drug effects, Arabidopsis - genetics, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Brassinosteroids - pharmacology, Carrier Proteins - genetics, Carrier Proteins - metabolism, F-Box Proteins - genetics, F-Box Proteins - metabolism, Gene Expression Regulation, Plant - drug effects, Gene Expression Regulation, Plant - genetics, Hypocotyl - drug effects, Hypocotyl - growth & development, Indoleacetic Acids - metabolism, Indoleacetic Acids - pharmacology, Microtubules - drug effects, Microtubules - genetics, Microtubules - metabolism, Mutation, Picloram - pharmacology, Plant Growth Regulators - metabolism, Plant Growth Regulators - pharmacology, Plants, Genetically Modified, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Seedlings - drug effects, Seedlings - metabolism, Signal Transduction - drug effects