Plant physiology (Bethesda), 2016-06, Vol.171 (2), p.1470-1484
2016
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Details
- Autor(en) / Beteiligte
- Titel
- The Raf-like Kinase ILK1 and the High Affinity K⁺ Transporter HAK5 Are Required for Innate Immunity and Abiotic Stress Response
- Ist Teil von
- Plant physiology (Bethesda), 2016-06, Vol.171 (2), p.1470-1484
- Ort / Verlag
- United States: American Society of Plant Biologists
- Erscheinungsjahr
- 2016
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Plant perception of pathogen-associated molecular patterns (PAMPs) and other environmental stresses trigger transient ion fluxes at the plasma membrane. Apart from the role of Ca²⁺ uptake in signaling, the regulation and significance of PAMP-induced ion fluxes in immunity remain unknown. We characterized the functions of INTEGRIN-LINKED KINASE1 (ILK1) that encodes a Raf-like MAP2K kinase with functions insufficiently understood in plants. Analysis of ILK1 mutants impaired in the expression or kinase activity revealed that ILK1 contributes to plant defense to bacterial pathogens, osmotic stress sensitivity, and cellular responses and total ion accumulation in the plant upon treatment with a bacterial-derived PAMP, flg22. The calmodulin-like protein CML9, a negative modulator of flg22-triggered immunity, interacted with, and suppressed ILK1 kinase activity. ILK1 interacted with and promoted the accumulation of HAK5, a putative (H⁺)/K⁺ symporter that mediates a high-affinity uptake during K⁺ deficiency. ILK1 or HAK5 expression was required for several flg22 responses including gene induction, growth arrest, and plasma membrane depolarization. Furthermore, flg22 treatment induced a rapid K⁺ efflux at both the plant and cellular levels in wild type, while mutants with impaired ILK1 or HAK5 expression exhibited a comparatively increased K⁺ loss. Taken together, our results position ILK1 as a link between plant defense pathways and K⁺ homeostasis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.16.00035Titel-ID: cdi_crossref_primary_10_1104_pp_16_00035
- Format
- –
- Schlagworte
- Amino Acid Sequence, Arabidopsis - genetics, Arabidopsis - immunology, Arabidopsis - microbiology, Arabidopsis - physiology, Arabidopsis Proteins - metabolism, Calmodulin - metabolism, Flagellin - pharmacology, Homeostasis - drug effects, Immunity, Innate - drug effects, Ions, Mannitol - pharmacology, Models, Biological, Mutation - genetics, Nicotiana - genetics, Osmosis - drug effects, Plant Diseases - immunology, Plant Diseases - microbiology, Plant Immunity - drug effects, Plants, Genetically Modified, Potassium - metabolism, Potassium-Hydrogen Antiporters - metabolism, Protein Binding - drug effects, Protein Serine-Threonine Kinases - chemistry, Protein Serine-Threonine Kinases - metabolism, Protein Transport - drug effects, Signal Transduction - drug effects, SIGNALING AND RESPONSE, Sodium Chloride - pharmacology, Stress, Physiological - drug effects, Subcellular Fractions - drug effects, Subcellular Fractions - metabolism