Plant physiology (Bethesda), 2012-03, Vol.158 (3), p.1463-1474
2012
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- Autor(en) / Beteiligte
- Titel
- TsHKT1;2, a HKT1 Homolog from the Extremophile Arabidopsis Relative Thellungiella salsuginea, Shows K+ Specificity in the Presence of NaCl
- Ist Teil von
- Plant physiology (Bethesda), 2012-03, Vol.158 (3), p.1463-1474
- Ort / Verlag
- Rockville, MD: American Society of Plant Biologists
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cellular Na(+)/K(+) ratio is a crucial parameter determining plant salinity stress resistance. We tested the function of plasma membrane Na(+)/K(+) cotransporters in the High-affinity K(+) Transporter (HKT) family from the halophytic Arabidopsis (Arabidopsis thaliana) relative Thellungiella salsuginea. T. salsuginea contains at least two HKT genes. TsHKT1;1 is expressed at very low levels, while the abundant TsHKT1;2 is transcriptionally strongly up-regulated by salt stress. TsHKT-based RNA interference in T. salsuginea resulted in Na(+) sensitivity and K(+) deficiency. The athkt1 mutant lines overexpressing TsHKT1;2 proved less sensitive to Na(+) and showed less K(+) deficiency than lines overexpressing AtHKT1. TsHKT1;2 ectopically expressed in yeast mutants lacking Na(+) or K(+) transporters revealed strong K(+) transporter activity and selectivity for K(+) over Na(+). Altering two amino acid residues in TsHKT1;2 to mimic the AtHKT1 sequence resulted in enhanced sodium uptake and loss of the TsHKT1;2 intrinsic K(+) transporter activity. We consider the maintenance of K(+) uptake through TsHKT1;2 under salt stress an important component supporting the halophytic lifestyle of T. salsuginea.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889, 1532-2548eISSN: 1532-2548DOI: 10.1104/pp.111.193110Titel-ID: cdi_crossref_primary_10_1104_pp_111_193110
- Format
- –
- Schlagworte
- Amino Acid Sequence, Arabidopsis - drug effects, Arabidopsis - genetics, Arabidopsis - physiology, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Biological and medical sciences, Biological Transport, Brassicaceae - drug effects, Brassicaceae - genetics, Brassicaceae - physiology, Cation Transport Proteins - genetics, Cation Transport Proteins - metabolism, Evolution, Molecular, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Plant, Genes, Plant, Homeostasis, Molecular Sequence Data, Mutagenesis, Site-Directed, Phylogeny, Plant physiology and development, Plant Roots - genetics, Plant Roots - physiology, Plants, Genetically Modified - drug effects, Plants, Genetically Modified - genetics, Plants, Genetically Modified - physiology, Potassium - metabolism, RNA Interference, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Salt-Tolerant Plants - drug effects, Salt-Tolerant Plants - genetics, Salt-Tolerant Plants - physiology, Sodium - metabolism, Sodium Chloride - pharmacology, Species Specificity, Substrate Specificity, Symporters - genetics, Symporters - metabolism