Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Ergebnis 20 von 274

Details

Autor(en) / Beteiligte
Titel
Protein-protein interaction panel using mouse full-length cDNAs
Ist Teil von
  • Genome research, 2001-10, Vol.11 (10), p.1758-1765
Ort / Verlag
United States
Erscheinungsjahr
2001
Quelle
MEDLINE
Beschreibungen/Notizen
  • We have developed a novel assay system for systematic analysis of protein-protein interactions (PPIs) that is characteristic of a PCR-mediated rapid sample preparation and a high-throughput assay system based on the mammalian two-hybrid method. Using gene-specific primers, we successfully constructed the assay samples by two rounds of PCR with up to 3.6 kb from the first-round PCR fragments. In the assay system, we designed all the steps to be performed by adding only samples, reagents, and cells into 384-well assay plates using two types of semiautomatic multiple dispensers. The system enabled us examine more than 20,000 assay wells per day. We detected 145 interactions in our pilot study using 3500 samples derived from mouse full-length enriched cDNAs. Analysis of the interaction data showed both several significant interaction clusters and predicted functions of a few uncharacterized proteins. In combination with our comprehensive mouse full-length cDNA clone bank covering a large part of the whole genes, our high-throughput assay system will discover many interactions to facilitate understanding of the function of uncharacterized proteins and the molecular mechanism of crucial biological processes, and also enable completion of a rough draft of the entire PPI panel in certain cell types or tissues of mouse within a short time.
Sprache
Englisch
Identifikatoren
ISSN: 1088-9051
eISSN: 1549-5469
DOI: 10.1101/gr.180101
Titel-ID: cdi_crossref_primary_10_1101_gr_180101

Weiterführende Literatur

Empfehlungen zum selben Thema automatisch vorgeschlagen von bX