Details

Autor(en) / Beteiligte

• Zaunders, John
• Munier, C. Mee Ling
• McGuire, Helen M
• Law, Hannah
• Howe, Annett
• Xu, Yin
• de St Groth, Barbara Fazekas
• Schofield, Peter
• Christ, Daniel
• Milner, Brad
• Obeid, Solange
• Dyer, Wayne B
• Saksena, Nitin K
• Kelleher, Anthony D

Titel

Mapping the extent of heterogeneity of human CCR5+ CD4+ T cells in peripheral blood and lymph nodes

Ist Teil von

• AIDS (London), 2020-05, Vol.34 (6), p.833-848

Ort / Verlag

England: Copyright Wolters Kluwer Health, Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• BACKGROUND:CD4 T cells that express the chemokine receptor, CCR5, are the most important target of HIV-1 infection, but their functions, phenotypes and anatomical locations are poorly understood. We aimed to use multiparameter flow cytometry to better define the full breadth of these cells. METHODS:High-parameter fluorescence flow and mass cytometry were optimized to analyse subsets of CCR5 memory CD4 T cells, including CD25CD127 Tregs, CXCR3CCR6− Th1-like, CCR6CD161CXCR3− Th17-like, integrins α4ß7 gut-homing, CCR4 skin-homing, CD62L lymph node-homing, CD38HLA-DR activated cells, and CD27−CD28− cytotoxic T lymphocytes, in a total of 22 samples of peripheral blood, ultrasound-guided fine needle biopsies of lymph nodes and excised tonsils. CCR5 antigen-specific CD4 T cells were studied using the OX40 flow-based assay. RESULTS:10–20% of CCR5 memory CD4 T cells were Tregs, 10–30% were gut-homing, 10–30% were skin-homing, 20–40% were lymph node-homing, 20–50% were Th1-like and 20–40% were Th17-like cells. Up to 30% were cytotoxic T lymphocytes in CMV-seropositive donors, including cells that were either CCR5Granzyme K or CCR5Granzyme B. When all possible phenotypes were exhaustively analysed, more than 150 different functional and trafficking subsets of CCR5 CD4 T cells were seen. Moreover, a small population of resident CD69Granzyme KCCR5 CD4 T cells was found in lymphoid tissues. CMV− and Mycobacterium tuberculosis-specific CD4 T cells were predominantly CCR5. CONCLUSION:These results reveal for the first time the prodigious heterogeneity of function and trafficking of CCR5 CD4 T cells in blood and in lymphoid tissue, with significant implications for rational approaches to prophylaxis for HIV-1 infection and for purging of the HIV-1 reservoir in those participants already infected.