Details

Autor(en) / Beteiligte

• Echejoh, Godwins
• Osborne, Aileen
• Miedzybrodzka, Zosia
• Macfarlane, Tatiana

Titel

Case-control study of epidermal growth factor receptor (EGFR) gene in head and neck cancer (HNC)

Ist Teil von

• Pathology, 2014, Vol.46, p.S92-S92

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The first intron of EGFR gene contains a microsatellite sequence of 9 to 23 CA-dinucleotide repeats that is very polymorphic. Transcription of the gene is inversely proportional to number of CA-repeats. Shorter CA-dinucleotide repeats cause over expression of EGFR mRNA and protein products with increased proliferation and survival advantage to the cells. Increased EGFR expression is reported to be associated with HNC susceptibility with poorer prognosis, and polymorphism in the CA-repeats is one of the early genetic alterations in HNC. We extracted DNA from blood and saliva samples from 51 (26 cases, 25 controls) participants of Feasibility Study of Cancer of the Head and Neck, Genetics and Environment in Grampian, and analysed using PCR and ABI 3130×L Genetic Analyser. Subjects’ ages ranged from 45-84 years; mean (SD) 63.15 ± 10.46 years. The EGFR risk association with HNC was calculated based on two categories: subjects with short CA-repeats (alleles ≤ 119bp) and those with long CA-repeats (alleles >119bp). After multivariate analysis adjustment for gender, tobacco, alcohol, and diet, there was increased risk of the short CA-dinucleotide repeats for HNC (OR= 1.50, 95% CI 0.34-6.71, p=0.6). The low statistical power is likely due to small sample size. However, the result agrees with what obtains in the literature database.