Details

Autor(en) / Beteiligte

• Surratt, Christopher K
• Wang, Jia-Bei
• Yuhasz, Stacieann
• Amzel, Mario
• Kwon, H Moo
• Handler, Joseph S
• Uhl, George R

Titel

Sodium- and chloride-dependent transporters in brain, kidney, and gut: lessons from complementary DNA cloning and structure-function studies

Ist Teil von

• Current opinion in nephrology and hypertension, 1993-09, Vol.2 (5), p.744-760

Ort / Verlag

England: Lippincott-Raven Publishers

Erscheinungsjahr

1993

Quelle

MEDLINE

Beschreibungen/Notizen

• The family of Na- and Cl-dependent, 12 transmembrane domain transporter proteins now includes transporters for neuretransmitter molecules in the brain and for substances important in extraneuronal tissues, including adrenal, kidney, and gut. Transported substrates include monoamine and amino acid neuretransmitters and nonper-turbing osmolytes. A common protein topology is predicted and features intracellular N- and C-termini possessing phosphorylation sites and at least one large extramembranous loop with N-linked glycosylation. Using the rat dopamine transporter as a template, molecular modeling of putative transmembrane domains coupled with amino acid sequence conservation analysis indicates amino acid residues potentially involved in substrate and/or ion recognition. Targeting such residues with site-directed mutagenesis will help clarify substrate and ion binding sites and should facilitate rational design of therapeutics to combat depression, locomotor disorders, and substance abuse.