Details

Autor(en) / Beteiligte

• Lala, Tanmoy Das
• Haag, Maria T
• Lafontant, Pascal J

Titel

Cardiomyocyte Cell Cycle Activity and Nucleation in the Regenerating Giant Danio Heart

Ist Teil von

• The FASEB journal, 2012-04, Vol.26 (S1)

Erscheinungsjahr

2012

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Abstract only Studies to date suggest that regeneration of the zebrafish heart after apical resection is supported by the proliferation of de‐differentiated cardiac myocytes (CMs). We developed a model of heart regeneration in response to cautery injury in Giant danio (GD), a closely related species to the zebrafish. We have previously shown that robust 5‐EDU incorporation in MEF‐positive cells in the regenerating compact heart and adjacent trabeculae, indicative of cell cycle re‐entry in CMs. Here we demonstrate by transmission electron microscopy that partially differentiated and mitotic CMs are present in the regenerating myocardium. To determine whether mitotic CMs proceeds through both karyokinesis and cytokinesis, we have performed nucleation studies on isolated CMs following enzymatic digestion. A sampling of 5000 CMs revealed an absence of bi‐nucleated cells in control or fully regenerated hearts, suggesting that cell cycle activity results in both karyokinesis and cytokinesis and the formation of new mono‐nucleated CMs, as opposed to the karyokinesis without cytokinesis reported in sub‐populations of adult mammalian ventricular CMs. Taken together, our data suggest that mono‐nucleation may be the terminal state of CMs in non‐injured and fully regenerated fish ventricular myocardium.