Details

Autor(en) / Beteiligte

• Apanavicius, Carolyn J.
• Vester, Brittany M.
• Karr‐Lilienthal, Lisa K.
• Pope, Lynda L.
• Swanson, Kelly S.

Titel

Effects of prebiotic supplementation on immune response to a bacterial challenge in weanling puppies

Ist Teil von

• The FASEB journal, 2006-03, Vol.20 (4), p.A172-A172

Ort / Verlag

Federation of American Societies for Experimental Biology

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Due to various stressors, weanling puppies are at high risk for illness if exposed to salmonella. Our objective was to evaluate the effect of fructan consumption on immune response to salmonella challenge in weanling puppies. A 2 x 3 factorial design was used. 30 hound‐cross puppies (12 wk‐old) were randomly assigned to three diets containing: 1) no prebiotic (control); 2) 1% short‐chain fructooligosaccharides (scFOS); or 3) 1% inulin. After 14 d on diet, dogs received an oral gavage of: 1) S. typhimurium DT104 (5x108 CFU) or 2) 0.9% saline (n = 5 per treatment). Vitals and activity scores were measured every 8 h for 48 h post‐infection. Blood was collected before and after infection (24 h; 48 h) for CBC and serum immunoglobulins. Intestinal samples were collected 48h post‐infection for mucosal cytokine gene expression, histomorphology, and pathology measurements. Data were compared using the mixed models procedure of SAS using treatment, infection, and treatment*infection as fixed effects and block as a random effect. Infected control dogs had a higher incidence of fever (>103°F) than infected dogs fed inulin. Neutrophil concentrations increased in all infected dogs, but the increase tended (P = 0.10) to be lower in dogs fed inulin or scFOS. Our results suggest that prebiotics may play attenuate the inflammatory response to infection in puppies. This research was funded in part by USDA‐CSREES (Project #ILLU‐538‐396).