Plant and cell physiology, 1990, Vol.31 (8), p.1177-1182
1990
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Details
- Autor(en) / Beteiligte
- Titel
- Induction of NADPH-cytochrome P-450 (c) reductase in wounded tissues from Helianthus tuberosus tubers
- Ist Teil von
- Plant and cell physiology, 1990, Vol.31 (8), p.1177-1182
- Ort / Verlag
- Oxford: Oxford University Press
- Erscheinungsjahr
- 1990
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- Cytochrome P-450 is not self-sufficient for the catalysis of monooxygenase reaction but requires NADPH and NADPH-cytochrome P-450 (c) reductase. The activity of NADPH-cyto-chrome P-450 reductase was strongly enhanced by wounding and aging in Jerusalem artichoke (Helianthus tuberosus L.) tuber tissues. This stimulation was correlated with the synthesis of the enzyme protein based on i) quantitation of the reductase protein by Western blotting, ii) incorporation of [35S]methionine into the immunoprecipitable enzyme and iii) an increase in translatable mRNA for the reductase in a cell free system.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0781, 1471-9053eISSN: 1471-9053DOI: 10.1093/oxfordjournals.pcp.a078032Titel-ID: cdi_crossref_primary_10_1093_oxfordjournals_pcp_a078032
- Format
- –
- Schlagworte
- Biological and medical sciences, CITOCROMO P 450, CYTOCHROME P 450, Enzymes, Fundamental and applied biological sciences. Psychology, HELIANTHUS TUBEROSUS, HERIDAS, Immunoprecipitation, In vitro translation, Metabolism, NADPH-cytochrome P450 (c) reductase, OXIDOREDUCTASES, OXIDORREDUCTASAS, OXYDOREDUCTASE, PLAIE, Plant physiology and development, PLANT TISSUES, TEJIDOS VEGETALES, TISSU VEGETAL, Western blot, WOUNDS