Details

Autor(en) / Beteiligte

• Fokina, Alesya
• Novopashina, Daria
• Meschaninova, Mariya
• Vorobjeva, Maria
• Zenkova, Marina
• Francois, Jean-Christophe
• Venyaminova, Alya

Titel

Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3′-odified oligo(2′-O-methylribonucleotide)-effectors

Ist Teil von

• Nucleic Acids Symposium Series, 2008-09, Vol.52 (1), p.525-526

Ort / Verlag

Oxford University Press

Erscheinungsjahr

2008

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Abstract Catalytic activity of DNAzymes targeted to IGF-I and MDR1 mRNA can be regulated by the combination of 10-23 DNAzyme with 3′-modified oligo(2′-O-methylribonucleotides) that are favorable as effectors due to their high affinity to RNA and nuclease resistance. We have demonstrated that the DNAzyme constructions designed were able to bind and cleave long structured RNA transcripts effectively under simulated physiological conditions.