Details

Autor(en) / Beteiligte

• Koekemoer, T C
• Downing, T G
• Oelofsen, W

Titel

An alternative PCR assay for quantifying mitochondrial DNA in crude preparations

Ist Teil von

• Nucleic acids research, 1998-06, Vol.26 (11), p.2829-2830

Ort / Verlag

England

Erscheinungsjahr

1998

Quelle

MEDLINE

Beschreibungen/Notizen

• A method is described for the quantification of mitochondrial DNA present in crude biological preparations. A known copy number of a standard is amplified in the presence of inactivated target DNA so as to determine the overall efficiency of the PCR process in a particular sample. In this way any inhibitory and/or stimulatory substances present in sample preparations can be taken into account. To reduce tube-to-tube variations product DNA quantification is limited to small cycle numbers. Using this method quantitations of DNA amounts in different crude preparations can be compared.