Details

Autor(en) / Beteiligte

• Costamagna, M E
• Cabanillas, A M
• Coleoni, A H
• Pellizas, C G
• Masini-Repiso, A M

Titel

Nitric Oxide Donors Inhibit Iodide Transport and Organification and Induce Morphological Changes in Cultured Bovine Thyroid Cells

Ist Teil von

• Thyroid (New York, N.Y.), 1998-12, Vol.8 (12), p.1127-1135

Ort / Verlag

United States

Erscheinungsjahr

1998

Quelle

MEDLINE

Beschreibungen/Notizen

• Nitric oxide (NO) has been proposed as an intracellular signal in the thyroid. The NO effect on function and morphology of bovine thyroid follicles in culture was analyzed by using the NO donors sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO). Both NO donors induced a concentration-dependent NO release measured by the nitrite accumulation in the culture medium. The SNP (10 to 500 μmol/L) treatment for 24 hours significantly inhibited the uptake, organification and transport of iodide in a concentration-dependent manner. When SNP (50 μmol/L) was withdrawn from the culture medium after 24 hours' incubation, iodide uptake and organification were partially recovered at 24 hours and reached the control value at 48 hours, indicating a reversible effect of SNP. A possible involvement of cyanide in the SNP inhibitory effect was excluded because incubation of follicles with potassium cyanide (KCN) at concentrations estimated to be present in the medium (40 and 80 μmol/L) for 24 hours did not modify iodide uptake and organification. The GSNO (10 to 500 μmol/L) treatment for 24 hours also reduced the iodide uptake, organification and transport in a concentration-dependent manner. A significant inhibition of iodide organification was induced after incubation with 1000 μmol/L of N 2 , 2′-O-dibutyrylguanosine 3′:5′-cyclic monophosphate ([Bu] 2 cGMP). Morphological evaluation by light microscopy revealed that the incubation with NPS or GSNO (500 μmol/L) produced cellular dispersion with loss of follicular cell aggregates that was evident at 96 hours exposure. Cell viability was not altered by 10-500 μmol/L SNP or GSNO (80% to 85%). We concluded that long-term NO exposure induces functional and morphological modifications compatible with a loss of differentiation in thyroid follicles. These observations further support a role of NO in the regulation of the thyroid function.