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Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Orbital Adipose/Connective Tissues is Increased During the Active Stage of Graves' Ophthalmopathy
Ist Teil von
Thyroid (New York, N.Y.), 2003-09, Vol.13 (9), p.845-850
Ort / Verlag
United States: Mary Ann Liebert, Inc
Erscheinungsjahr
2003
Quelle
MEDLINE
Beschreibungen/Notizen
The mechanisms involved in the increase of orbital retro-ocular adipose tissue that occurs in Graves' ophthalmopathy (GO) are still unclear. In this condition, the orbital tissue shows glycosaminoglycans
deposition produced by activated fibroblasts capable of undergoing adipocytic differentiation. Many genes are involved in adipogenic mechanisms including the transcription factor peroxisome proliferator-activated
receptor-γ (PPAR-γ). We evaluated the level of expression of the PPAR-γ gene in normal and GO orbital adipose/connective tissue specimens using a quantitative and sensitive reverse transcription
(RT) competitive polymerase chain reaction (PCR) assay. Our results show that the expression of PPAR-γ was significantly greater in adipose/connective tissue from patients in the active stage of GO
than in controls (150.8 ± 103.9 and 24.0 ± 4.9 amol/micro g of total RNA respectively,
p
< 0.05), while there was no significant difference between patients with inactive GO (58.8
± 40.6 aM/
μ
g total RNA) and controls. These results suggest that increased PPAR-γ gene expression in the active stage of GO may be dependent on the inflammatory process in this disease.
We speculate that the increased orbital fat tissue observed in GO may be a consequence of the anti-inflammatory PPAR-γ action.