Artificial cells, nanomedicine, and biotechnology, 2020-01, Vol.48 (1), p.777-788
2020
Details
- Autor(en) / Beteiligte
- Titel
- Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages
- Ist Teil von
- Artificial cells, nanomedicine, and biotechnology, 2020-01, Vol.48 (1), p.777-788
- Ort / Verlag
- England: Taylor & Francis
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Probiotic Gluconacetobacter strains are intestinal microbes with beneficial effects on human health. Recently, researchers have used these strains to biosynthesize metal and non-metal nanoparticles for treating various chronic diseases. Despite their importance in nanotechnology, gold nanoparticles (AuNPs) biosynthesized by Gluconacetobacter species have not been clearly identified for treating inflammation and inflammation-associated diseases. While ginsenoside CK has strong pharmaceutical activity, it also has strong cytotoxicity and hydrophobicity which is hurdle to make formulation. Peptide-nanoparticle hybrids are gaining increasing attention for their potential biomedical applications, including human inflammatory diseases. Herein, we developed peptide CopA3 surface conjugated and ginsenoside compound K (CK) loaded gold nanoparticles (GNP-CK-CopA3), which intracellularly synthesised by the probiotic Gluconacetobacter liquefaciens kh-1, to target lipopolysaccharide (LPS)-activated RAW264.7 macrophages. The synthetic GNP-CK-CopA3 was characterised by various instrumental techniques. The results of our cellular uptake and MTT assays exhibited obvious drug intracellular delivery without significant cytotoxicity. In addition, pre-treatment with GNP-CK-CopA3 significantly ameliorated LPS-induced nitric oxide (NO) and reactive oxygen species (ROS) production and suppressed the mRNA and protein expression of pro-inflammatory cytokines in macrophages. Furthermore, GNP-CK-CopA3 efficiently inhibited the activation of the nuclear factor-κB (NF-κB) and mitogen-activating protein kinase (MAPK) signalling pathways. Taken together, our findings highlight the potential of using peptide-nanoparticle hybrids in the development of anti-inflammatory approaches and providing the experimental foundation for further application.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2169-1401eISSN: 2169-141XDOI: 10.1080/21691401.2020.1748639Titel-ID: cdi_crossref_primary_10_1080_21691401_2020_1748639
- Format
- –
- Schlagworte
- Animals, anti-inflammatory, Anti-Inflammatory Agents - chemistry, Anti-Inflammatory Agents - pharmacology, Antimicrobial Cationic Peptides - chemistry, Antimicrobial Cationic Peptides - pharmacology, Biocompatibility, Biomedical materials, Cell Line, Compound K, Cytokines, Cytokines - genetics, Cytokines - metabolism, Cytotoxicity, Drug Delivery Systems, Gene expression, Ginsenosides, Ginsenosides - chemistry, Ginsenosides - pharmacology, Gluconacetobacter - metabolism, Gold, Gold - chemistry, Gold - pharmacology, gold nanoparticles, Humans, Hybrids, Hydrophobicity, Inflammation, Inflammatory diseases, Insect Proteins - chemistry, Insect Proteins - pharmacology, Intestine, Intracellular, Kinases, Lipopolysaccharides, Lipopolysaccharides - pharmacology, Macrophages, Macrophages - drug effects, Macrophages - metabolism, MAP kinase, Metal Nanoparticles - chemistry, Mice, Mitogen-Activated Protein Kinases - metabolism, mRNA, Nanoparticles, Nanotechnology, NF-kappa B - metabolism, NF-κB, NF-κB protein, Nitric oxide, Nitric Oxide - metabolism, Peptide CopA3, Peptides, Pretreatment, Probiotics, Protein kinase, Proteins, Reactive oxygen species, Reactive Oxygen Species - metabolism, Signal transduction, Signal Transduction - drug effects, Toxicity