Details

Autor(en) / Beteiligte

• Pedersen, B.H

Titel

DNA fingerprints of 51 sweet and sour Prunus accessions using Simple Sequence Repeats

Ist Teil von

• The journal of horticultural science & biotechnology, 2006, Vol.81 (1), p.118-124

Ort / Verlag

Taylor & Francis

Erscheinungsjahr

2006

Quelle

Taylor & Francis

Beschreibungen/Notizen

• Ten previously described PCR primer pairs were tested on 51 sweet and sour cherry accessions to determine if the primers could differentiate between accessions. The SSR fragment scores were high, with only a few null loci observed for all primer/accession combinations. The loci were highly polymorphic, with 14 - 40 different alleles found at each locus. Heterozygosity values ranged from 0.52 - 1.00, gene diversity (PIC) values ranged from 0.905 - 0.966, and the discriminating power of each locus varied from 0.896 - 0.965 for the sour cherry accessions, from 0.48 - 0.967 for the sweet cherry genotypes, and from 0.803 - 0.921 for the cherry rootstocks. The combined discriminating power of all loci was effectively 1.0. Two accessions shared the same DNA-profile, the sour cherry cultivar 'Zigeuner Kirschen' (accession no. DKPcC429) and the cherry rootstock 'Weiroot 13' (accession no. DKPcR49). These genotypes are considered to be identical. In this investigation, however, it was not possible to achieve identical DNA-fingerprints for seven sour cherry accessions held at the Danish Research Centre compared to cultivars with the same names from the collection at the U.S. Department of Agriculture, Agricultural Research Service Geneva, NY.