Details

Autor(en) / Beteiligte

• Wang, Wenwen
• Foerner, Elena
• Buss, Eike
• Jauch, Anna
• Eckstein, Volker
• Wuchter, Patrick
• Ho, Anthony D.
• Lutz, Christoph

Titel

Hematopoietic stem cells can be separated from leukemic cells in a subgroup of adult acute lymphoblastic leukemia patients

Ist Teil von

• Leukemia & lymphoma, 2017-06, Vol.58 (6), p.1446-1454

Ort / Verlag

United States: Taylor & Francis

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• In B-cell acute lymphoblastic leukemia (B-ALL) separation of normal hematopoietic stem cells (HSC) has so far been limited to a subgroup of patients. As aldehyde dehydrogenase (ALDH)-activity is enriched in various stem cells we investigated its value for HSC isolation in adult B-ALL. Based on ALDH-activity patients could be stratified in ALDH-numerous (≥1.9% ALDH +  cells) and ALDH-rare (<1.9% ALDH +  cells) cases. In ALDH-rare B-ALL clonal-marker negative HSC could be separated by the CD34 + CD38 − ALDH +  phenotype, whereas this separation was not possible in ALDH-numerous B-ALL. Functional analysis confirmed the HSC-potential of isolated cells, which were uniformly CD19-negative. However, addition of ALDH-activity further improved HSC-purity. In summary, we provide a method to separate functionally normal HSC from leukemic cells in a subgroup of B-ALL patients that can be identified prospectively. This protocol thereby facilitates comparative analyses of matched HSC and leukemic cells in order to improve our understanding of leukemia evolution.