Details

Autor(en) / Beteiligte

• Pereira, Lívia Maria Galdino
• Andrade, Ana Raquel Colares de
• Portela, Fernando Victor Monteiro
• Aguiar, Ana Luiza Ribeiro
• Silva, Bruno Nascimento da
• Moura, Santiago Gonçalves Bezerra
• Pergentino, Mariana Lara Mendes
• Rocha, Marcos Fábio Gadelha
• Sidrim, José Júlio da Costa
• Castelo Branco, Débora de Souza Collares Maia
• Cordeiro, Rossana de Aguiar

Titel

Heterologous extracellular DNA facilitates the development of Trichosporon asahii and T. inkin biofilms and enhances their tolerance to antifungals

Ist Teil von

• Biofouling (Chur, Switzerland), 2022-09, Vol.38 (8), p.778-785

Ort / Verlag

Abingdon: Taylor & Francis

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Trichosporon asahii and T. inkin are emergent agents of deep-seated and disseminated infections in immunocompromised patients. The present study aimed to investigate the role of extracellular DNA (eDNA) and the enzyme deoxyribonuclease (DNase) on the structure of T. asahii and T. inkin biofilms, as well as to examine their effect on the susceptibility to antifungals. Biofilms reached maturity at 48 h; eDNA concentration in the supernatant increased over time (6 < 24 h < 48h). Exogenous eDNA increased biomass of Trichosporon biofilms at all stages of development, enhanced their tolerance to antifungals and improved their structural complexity. DNase reduced biomass, biovolume and thickness of Trichosporon biofilms, thereby rendering them more susceptibility to voriconazole. The results suggest the relevance of eDNA in the structure and antifungal susceptibility of Trichosporon biofilms and highlight the potential of DNase as adjuvant in biofilm control.