Details

Autor(en) / Beteiligte

• Shimizu, Hiroko
• Uetsuka, Koji
• Nakayama, Hiroyuki
• Doi, Kunio

Titel

Carbon tetrachloride-induced acute liver injury in Mini and Wistar rats

Ist Teil von

• Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2001, Vol.53 (1), p.11-18

Ort / Verlag

Jena: Elsevier GmbH

Erscheinungsjahr

2001

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Acute liver injury induced by CCl 4 injection (0.5 ml/kg b.w.) was compared between Mini and Wistar rats. Mini rats (Jcl:Wistar-TgN (ARGHGEN)1Nts strain) are Wistar-derived transgenic animals in which the expression of growth hormone (GH) gene is suppressed by the presence of an antisense transgene. The hepatic lesion appeared earlier and its recovery was delayed in Mini rats compared to in Wistar rats. The degree of the liver injury was more severe in Mini rats than in Wistar rats, and this corresponded well with the changes in serum AST level. Moreover, in accordance with the localization of CYP2E1-positive hepatocytes in the early stage after CCl 4 treatment, the initial lesion characterized by ballooning of hepatocytes developed in the centrilobular zone in Wistar rats while it appeared in the middle zone in Mini rats. The changes in the percentage of PCNA-positive cells and the levels of HGF and TGF-β1 mRNAs were clearly different between the two strains. These results indicate that the response of the liver to CCl 4 is different between GH-suppressed Mini rats and Wistar rats.