Details

Autor(en) / Beteiligte

• Martinez, Xavier
• Brandt, Christian
• Saddallah, Fatiha
• Tougne, Chantal
• Barrios, Christy
• Wild, Fabian
• Dougan, Gordan
• Lambert, Paul-Henri
• Siegrist, Claire-Anne

Titel

DNA Immunization Circumvents Deficient Induction of T Helper Type 1 and Cytotoxic T Lymphocyte Responses in Neonates and during Early Life

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1997-08, Vol.94 (16), p.8726-8731

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• The relative deficiency of T helper type 1 (Th1) and cytotoxic T lymphocyte (CTL) responses in early life is associated with an increased susceptibility to infections by intracellular microorganisms. This is likely to reflect a preferential polarization of immature CD4 T cells toward a Th2 rather than a Th1 pattern upon immunization with conventional vaccines. In this report, it is shown that a single immunization within the first week of life with DNA plasmids encoding viral (measles virus hemagglutinin, Sendai virus nucleoprotein) or bacterial (C fragment of tetanus toxin) vaccine antigens can induce adult-like Th1 or mixed Th1/Th2 responses indicated by production of IgG2a vaccine-specific antibodies and preferential secretion of interferon-γ (IFN-γ ) compared with interleukin (IL)-5 by antigen-specific T cells, as well as significant CTL responses. However, in spite of this potent Th1-driving capacity, subsequent DNA immunization was not capable of reverting the Th2-biased responses induced after early priming with a recombinant measles canarypox vector. Thus, DNA vaccination represents a novel strategy capable of inducing Th1 or mixed Th1/Th2 and CTL responses in neonates and early life, providing it is performed prior to exposure to Th2-driving conventional vaccine antigens.