Details

Autor(en) / Beteiligte

• Foster, Donald C.
• Sprecher, Cindy A.
• Grant, Francis J.
• Kramer, Janet M.
• Kuijper, Joseph L.
• Holly, Richard D.
• Whitmore, Theodore E.
• Heipel, Mark D.
• Bell, L. Anne
• Andrew F. T. Ching
• McGrane, Vicki
• Hart, Charles
• O'Hara, Patrick J.
• Lok, Si

Titel

Human Thrombopoietin: Gene Structure, cDNA Sequence, Expression, and Chromosomal Localization

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1994-12, Vol.91 (26), p.13023-13027

Ort / Verlag

United States: National Academy of the Sciences of the United States of America

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• Thrombopoietin (TPO), a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Recently we have isolated a cDNA encoding a ligand for the murine c-mpl protooncogene and shown it to be TPO. By employing a murine cDNA probe, we have isolated a gene encoding human TPO from a human genomic library. The TPO locus spans over 6 kb and has a structure similar to that of the erythropoietin gene (EPO). Southern blot analysis of human genomic DNA reveals a hybridization pattern consistent with a single gene locus. The locus was mapped by in situ hybridization of metaphase chromosome preparations to chromosome 3q26-27, a site where a number of chromosomal abnormalities associated with thrombocythemia in cases of acute myeloid leukemia have been mapped. A human TPO cDNA was isolated by PCR from kidney mRNA. The cDNA encodes a protein with 80% identity to previously described murine TPO and is capable of initiating a proliferative signal to murine interleukin 3-dependent BaF3 cells expressing the murine or human TPO receptor.