Proceedings of the National Academy of Sciences - PNAS, 1992-01, Vol.89 (1), p.246-250
1992
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Details
- Autor(en) / Beteiligte
- Titel
- Determination of Ligand-Binding Specificity by Alternative Splicing: Two Distinct Growth Factor Receptors Encoded by a Single Gene
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1992-01, Vol.89 (1), p.246-250
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1992
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Expression cDNA cloning and structural analysis of the human keratinocyte growth factor receptor (KGFR) revealed identity with one of the fibroblast growth factor (FGF) receptors encoded by the bek gene (FGFR-2), except for a divergent stretch of 49 amino acids in their extracellular domains. Binding assays demonstrated that the KGFR was a high-affinity receptor for both KGF and acidic FGF, while FGFR-2 showed high affinity for basic and acidic FGF but no detectable binding by KGF. Genomic analysis of the bek gene revealed two alternative exons responsible for the region of divergence between the two receptors. The KGFR transcript was specific to epithelial cells, and it appeared to be differentially regulated with respect to the alternative FGFR-2 transcript. Thus, two growth factor receptors with different ligand-binding specificities and expression patterns are encoded by alternative transcripts of the same gene.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.89.1.246Titel-ID: cdi_crossref_primary_10_1073_pnas_89_1_246
- Format
- –
- Schlagworte
- 550201 - Biochemistry- Tracer Techniques, Amino Acid Sequence, Amino acids, ANIMALS, Base Sequence, BASIC BIOLOGICAL SCIENCES, BETA DECAY RADIOISOTOPES, Biochemistry, Biological and medical sciences, BODY, Cell receptors, Cell structures and functions, Cellular biology, Cloning, Molecular, Complementary DNA, DAYS LIVING RADIOISOTOPES, DNA, DNA - genetics, DNA POLYMERASES, DNA SEQUENCING, ELECTRON CAPTURE RADIOISOTOPES, ENZYMES, Epithelial cells, Exons, Fibroblast Growth Factor 10, Fibroblast Growth Factor 7, Fibroblast Growth Factors - metabolism, Fundamental and applied biological sciences. Psychology, Genes, Genetics, Growth factor receptors, GROWTH FACTORS, Growth Substances - metabolism, Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors, Humans, INTERMEDIATE MASS NUCLEI, INTERNAL CONVERSION RADIOISOTOPES, IODINE 125, IODINE ISOTOPES, ISOTOPE APPLICATIONS, ISOTOPES, KERATIN, Ligands, MAMMALS, MAN, Medical research, MEMBRANE PROTEINS, Mice, MITOGENS, Molecular and cellular biology, Molecular Sequence Data, NIH 3T3 cells, NUCLEI, NUCLEIC ACIDS, NUCLEOTIDYLTRANSFERASES, ODD-EVEN NUCLEI, Oligodeoxyribonucleotides - chemistry, ORGANIC COMPOUNDS, ORGANS, PHOSPHORUS-GROUP TRANSFERASES, PHOSPHOTRANSFERASES, Polymerase Chain Reaction, POLYMERASES, PRIMATES, PROTEINS, RADIOISOTOPES, RADIORECEPTOR ASSAY, Receptor, Fibroblast Growth Factor, Type 2, RECEPTORS, Receptors, Cell Surface - genetics, Receptors, Fibroblast Growth Factor, RECOMBINANT DNA, RNA Splicing, RNA, Messenger - genetics, SCLEROPROTEINS, Sequence Alignment, SKIN, splicing, STRUCTURAL CHEMICAL ANALYSIS, Structure-Activity Relationship, TRACER TECHNIQUES, TRANSFERASES, VERTEBRATES