Proceedings of the National Academy of Sciences - PNAS, 1988-04, Vol.85 (7), p.2334-2338
1988
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Details
- Autor(en) / Beteiligte
- Titel
- Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin from Bovine Aortic Endothelial Cells is Coupled
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1988-04, Vol.85 (7), p.2334-2338
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1988
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Bovine aortic endothelial cells were grown on microcarrier beads and were perfused with Krebs-Ringer solution. Endothelium-derived relaxing factor (EDRF) was bioassayed on a cascade of four strips of rabbit aorta, and prostacyclin was analyzed by RIA of 6-oxo-prostaglandin F1α. The endothelial cells released EDRF and prostacyclin when stimulated with bradykinin and its analogues, or with ADP, ATP, arachidonic acid, and phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3). The detection of EDRF was potentiated by superoxide dismutase, and the relaxation of rabbit aortic strips induced by EDRF was antagonized by methylene blue. The release of EDRF and prostacyclin was inhibited by phorbol myristate acetate, R59022 (a diacylglycerol kinase inhibitor), and gentamycin. We suggest that the release of EDRF and prostacyclin is coupled and the initial common step is activation of a phospholipase C.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.85.7.2334Titel-ID: cdi_crossref_primary_10_1073_pnas_85_7_2334
- Format
- –
- Schlagworte
- Agonists, Amines, ANIMAL TISSUES, ANIMALS, AORTA, Aorta - metabolism, ARTERIES, BASIC BIOLOGICAL SCIENCES, BIOASSAY, Biological and medical sciences, BIOLOGICAL EFFECTS, Biological Products - metabolism, BLOOD VESSELS, Blood vessels and receptors, BODY, CARCINOGENS, CARDIOVASCULAR SYSTEM, CATTLE, CHALCOGENIDES, CHEMICAL ACTIVATION, DIAGNOSTIC TECHNIQUES, Diglycerides, DOMESTIC ANIMALS, Endothelial cells, ENDOTHELIUM, Endothelium, Vascular - metabolism, Enzyme Activation, ENZYME INDUCTION, ENZYMES, Epoprostenol - metabolism, ESTERASES, ESTERS, Fundamental and applied biological sciences. Psychology, GENE REGULATION, Gentamicins - pharmacology, HYDROLASES, IMMUNOASSAY, IMMUNOLOGY, ISOTOPE APPLICATIONS, MAMMALS, Medical sciences, MEMBRANE PROTEINS, Methylene Blue - pharmacology, NITRIC OXIDE, Nitrites, NITROGEN COMPOUNDS, NITROGEN OXIDES, Nucleotides, ORGANIC COMPOUNDS, ORGANS, OXIDES, OXYGEN COMPOUNDS, PHORBOL ESTERS, Phorbols, PHOSPHODIESTERASES, PROSTAGLANDINS, PROTEINS, Pyrimidinones - pharmacology, RADIOASSAY, RADIOIMMUNOASSAY, RADIOIMMUNODETECTION, RADIOIMMUNOLOGY, RECEPTORS, Receptors, Cell Surface - drug effects, Receptors, Cell Surface - physiology, RUMINANTS, Superoxide Dismutase - pharmacology, Tetradecanoylphorbol Acetate - pharmacology, Thiazoles - pharmacology, TISSUES, TRACER TECHNIQUES, Type C Phospholipases - metabolism, Ungulates, VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques, Vertebrates: cardiovascular system