Details

Autor(en) / Beteiligte

• Rolle, Anna-Maria
• Hasenberg, Mike
• Thornton, Christopher R.
• Solouk-Saran, Djamschid
• Männ, Linda
• Weski, Juliane
• Maurer, Andreas
• Fischer, Eliane
• Spycher, Philipp R.
• Schibli, Roger
• Boschetti, Frederic
• Stegemann-Koniszewski, Sabine
• Bruder, Dunja
• Severin, Gregory W.
• Autenrieth, Stella E.
• Krappmann, Sven
• Davies, Genna
• Pichler, Bernd J.
• Gunzer, Matthias
• Wiehr, Stefan

Titel

ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2016-02, Vol.113 (8), p.E1026-E1033

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2016

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [64Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [64Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [18F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.