Details

Autor(en) / Beteiligte

• Zhang, Lianjun
• Chen, Min
• Wen, Qing
• Li, Yaqiong
• Wang, Yaqing
• Wang, Yanbo
• Qin, Yan
• Cui, Xiuhong
• Yang, Lin
• Huff, Vicki
• Gao, Fei

Titel

Reprogramming of Sertoli cells to fetal-like Leydig cells by Wt1 ablation

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2015-03, Vol.112 (13), p.4003-4008

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• Sertoli and Leydig cells, the two major somatic cell types in the testis, have different morphologies and functions. Both are essential for gonad development and spermatogenesis. However, whether these cells are derived from the same progenitor cells and the mechanism regulating the differentiation between these two cell types during gonad development remains unclear. A previous study showed that overactivation of C tnnb1 (cadherin-associated protein, beta 1) in Sertoli cells resulted in Sertoli cell tumors. Surprisingly, in the present study, we found that simultaneous deletion of Wilms’ Tumor Gene 1 ( Wt1 ) and overactivation of Ctnnb1 in Sertoli cells led to Leydig cell-like tumor development. Lineage tracing experiments revealed that the Leydig-like tumor cells were derived from Sertoli cells. Further studies confirmed that Wt1 is required for the maintenance of the Sertoli cell lineage and that deletion of Wt1 resulted in the reprogramming of Sertoli cells to Leydig cells. Consistent with this interpretation, overexpression of Wt1 in Leydig cells led to the up-regulation of Sertoli cell-specific gene expression and the down-regulation of steroidogenic gene expression. These results demonstrate that the distinction between Sertoli cells and Leydig cells is regulated by Wt1 , implying that these two cell types most likely originate from the same progenitor cells. This study thus provides a novel concept for somatic cell fate determination in testis development that may also represent an etiology of male infertility in human patients. Significance Genetic control of the differentiation between Sertoli cells and granulosa cells has been reported previously. However, the relationship between Sertoli cells and Leydig cells in the testis has not yet been definitively determined. In the present study, we demonstrate for the first time, to our knowledge, that these two cell types can be mutually reprogrammed and that Wilms’ Tumor Gene 1 ( Wt1 ) plays a critical role in this process. This study provides a novel concept for cell fate determination in testis development that will improve our understanding of the regulatory mechanisms of gonad development.