Proceedings of the National Academy of Sciences - PNAS, 2013-06, Vol.110 (24), p.9631-9631
2013
Details
- Autor(en) / Beteiligte
- Titel
- Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2013-06, Vol.110 (24), p.9631-9631
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1302877110Titel-ID: cdi_crossref_primary_10_1073_pnas_1302877110
- Format
- –
- Schlagworte
- Animals, Antimicrobial agents, Biological Sciences, Bone Marrow Cells - cytology, Bone Marrow Cells - immunology, Bone Marrow Cells - metabolism, Cell Differentiation - genetics, Cell Differentiation - immunology, Cell Proliferation - drug effects, Cells, Cells, Cultured, Cytokines, Cytokines - genetics, Cytokines - immunology, Cytokines - metabolism, Gram-positive bacteria, Granulocyte-Macrophage Colony-Stimulating Factor - immunology, Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology, Humans, Interleukin-1alpha - genetics, Interleukin-1alpha - immunology, Interleukin-1alpha - metabolism, Lipopolysaccharides - immunology, Lipopolysaccharides - pharmacology, Lungs, Macrophages - cytology, Macrophages - immunology, Macrophages - metabolism, Macrophages, Alveolar - cytology, Macrophages, Alveolar - immunology, Macrophages, Alveolar - metabolism, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Mycobacterium tuberculosis - immunology, Oligonucleotide Array Sequence Analysis, Phagocytosis - immunology, PNAS Plus, PNAS PLUS: SIGNIFICANCE STATEMENTS, Propionibacterium acnes - immunology, STAT5 Transcription Factor - genetics, STAT5 Transcription Factor - immunology, STAT5 Transcription Factor - metabolism, Tissues, Transcriptome - drug effects, Transcriptome - genetics, Transcriptome - immunology