Details

Autor(en) / Beteiligte

• Tresset, Guillaume
• Iliescu, Ciprian

Titel

Electrical control of loaded biomimetic femtoliter vesicles in microfluidic system

Ist Teil von

• Applied physics letters, 2007-04, Vol.90 (17), p.173901-173901-3

Ort / Verlag

American Institute of Physics

Erscheinungsjahr

2007

Quelle

美国小型学会期刊集（AIP Scitation平台）

Beschreibungen/Notizen

• This letter describes how femtoliter lipid vesicles encapsulating a fluorescent marker can be aligned, porated, and fused by electric field in microfluidic system. Vesicles are prepared with a soluble dye and filtrated through a size-exclusion column. After introduction into a microdevice comprising heavily doped silicon electrodes sealed between glass substrates, the vesicles are arranged in "pearl chain" clusters under the application of an external ac voltage. Release of the encapsulated dye upon the application of high electric field pulses reveals the formation of submicrometer pores in the membrane, and the fusion of distinct vesicles can also be achieved.