Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
β‐TUBULIN GENE OF PORPHYRA PURPUREA ( RHODOPHYTA)1
Ist Teil von
Journal of phycology, 2002-10, Vol.38 (5), p.1020-1023
Ort / Verlag
Boston, MA, USA: Blackwell Science Inc
Erscheinungsjahr
2002
Link zum Volltext
Quelle
Wiley Online Library
Beschreibungen/Notizen
The life cycle of the marine red alga Porphyra purpurea (Roth) C. Agardh includes a shell‐boring filamentous sporophyte and a leafy gametophyte. A single intronless gene for the microtubule protein β‐tubulin was discovered by molecular cloning of P. purpurea cDNA and genomic DNA. This gene, named TubB1, encodes a β‐tubulin with a divergent amino acid sequence, showing 74% identity with the conserved β‐tubulin of Chlamydomonas reinhardtii P. A. Dangeard. Southern hybridization analysis of nuclear DNA confirmed that P. purpurea has a single TubB1 gene. Transcripts (1.8 kb) of TubB1 are present in the sporophyte and gametophyte. Codon bias indicates strong expression of TubB1. The divergent nature of the TubB1 genes suggests that the absence of axonemal structures has allowed substantial genetic drift in red algal β‐tubulin genes.