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Details

Autor(en) / Beteiligte
Titel
Extremophilic behavior of catalytic amyloids sustained by backbone structuring
Ist Teil von
  • Journal of materials chemistry. B, Materials for biology and medicine, 2022-11, Vol.1 (45), p.94-9412
Ort / Verlag
England: Royal Society of Chemistry
Erscheinungsjahr
2022
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
  • Enzyme function relies on the placement of chemistry defined by solvent and self-associative hydrogen bonding displayed by the protein backbone. Amyloids, long-range multi-peptide and -protein materials, can mimic enzyme functions while having a high proportion of stable self-associative backbone hydrogen bonds. Though catalytic amyloid structures have exhibited a degree of temperature and solvent stability, defining their full extremophilic properties and the molecular basis for such extreme activity has yet to be realized. Here we demonstrate that, like thermophilic enzymes, catalytic amyloid activity persists across high temperatures with an optimum activity at 81 °C where they are 30-fold more active than at room temperature. Unlike thermophilic enzymes, catalytic amyloids retain both activity and structure well above 100 °C as well as in the presence of co-solvents. Changes in backbone vibrational states are resolved in situ using non-linear 2D infrared spectroscopy (2DIR) to reveal that activity is sustained by reorganized backbone hydrogen bonds in extreme environments, evidenced by an emergent vibrational mode centered at 1612 cm −1 . Restructuring also occurs in organic solvents, and facilitates complete retention of hydrolysis activity in co-solvents of lesser polarity. We support these findings with molecular modeling, where the displacement of water by co-solvents leads to shorter, less competitive, bonding lifetimes that further stabilize self-associative backbone interactions. Our work defines amyloid properties that counter classical proteins, where extreme environments induce mechanisms of restructuring to support enzyme-like functions necessary for synthetic applications. Catalytic amyloid materials remain structured in extreme temperature and solvent conditions, enabling enzyme-like function in synthetic environments.
Sprache
Englisch
Identifikatoren
ISSN: 2050-750X
eISSN: 2050-7518
DOI: 10.1039/d2tb01605b
Titel-ID: cdi_crossref_primary_10_1039_D2TB01605B

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