Details

Autor(en) / Beteiligte

• Zhang, Yan
• Li, Qing-nan
• Xiang, Dong-xue
• Zhou, Kaiyue
• Xu, Qinfeng
• Zhang, Chun-yang

Titel

Development of a bidirectional isothermal amplification strategy for the sensitive detection of transcription factors in cancer cells

Ist Teil von

• Chemical communications (Cambridge, England), 2020-08, Vol.56 (63), p.8952-8955

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• We developed a new strategy to sensitively detect transcription factors (TFs) based on the integration of a bidirectional isothermal exponential amplification reaction (EXPAR) with endonuclease IV (endo IV)-assisted cycle digestion of signal probes. This assay exhibits ultrahigh sensitivity with a detection limit of 1.29 × 10 −14 M, and it can measure endogenous NF-κB p50 in HeLa cell extracts. Moreover, this strategy can be applied to screen TF inhibitors and detect other TFs by simply changing the TF-binding sequence. We develop a bidirectional isothermal amplification strategy for the sensitive detection of transcription factors in cancer cells.