Details

Autor(en) / Beteiligte

• Wu, Yonghui
• Chang, Guanxiao
• Zhao, Yanbao
• Zhang, Yu

Titel

Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins

Ist Teil von

• Dalton transactions : an international journal of inorganic chemistry, 2014-01, Vol.43 (2), p.779-783

Ort / Verlag

England

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Hollow nickel silicate nanospheres (NiSiO 3 NSs) with hierarchical shells were hydrothermally synthesized by using silica spheres as a template. The NiSiO 3 NSs have an average diameter of 250 nm with a shell thickness of 50 nm, and the hierarchical shell consists of a large number of sheets. By taking advantage of the high affinity of Ni 2+ toward histidine-tagged (His-tagged) proteins, hollow NiSiO 3 NSs can be used to enrich and separate His-tagged proteins directly from a mixture of lysed cells. Results indicated that the hollow NiSiO 3 NSs presented negligible nonspecific protein adsorption and a high protein binding ability with a high binding capacity of 13.2 mmol g −1 . Their specificity and affinity toward His-tagged proteins remained after recycling 5 times. The hollow NiSiO 3 NSs are especially suitable for rapid purification of His-tagged proteins. Hollow NiSiO 3 nanospheres with hierarchical shells were used to selectively separate His-tagged proteins directly from the mixture of lysed cells.