Nature methods, 2020-03, Vol.17 (3), p.283-286
2020
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Details
- Autor(en) / Beteiligte
- Titel
- Simultaneous multiplane imaging with reverberation two-photon microscopy
- Ist Teil von
- Nature methods, 2020-03, Vol.17 (3), p.283-286
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/s41592-019-0728-9Titel-ID: cdi_crossref_primary_10_1038_s41592_019_0728_9
- Format
- –
- Schlagworte
- Acoustics, Animals, Brain - diagnostic imaging, Female, Imaging, Three-Dimensional, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, Multiphoton - methods, Motor Cortex - diagnostic imaging, Neurons - physiology, Olfactory Bulb - diagnostic imaging, Optics and Photonics, Phantoms, Imaging, Photons, Scattering, Radiation, Signal-To-Noise Ratio