Nature methods, 2019-08, Vol.16 (8), p.703-706
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Thesaurus: quantifying phosphopeptide positional isomers
- Ist Teil von
- Nature methods, 2019-08, Vol.16 (8), p.703-706
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Proteins can be phosphorylated at neighboring sites resulting in different functional states, and studying the regulation of these sites has been challenging. Here we present Thesaurus, a search engine that detects and quantifies phosphopeptide positional isomers from parallel reaction monitoring and data-independent acquisition mass spectrometry experiments. We apply Thesaurus to analyze phosphorylation events in the PI3K/AKT signaling pathway and show neighboring sites with distinct regulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/s41592-019-0498-4Titel-ID: cdi_crossref_primary_10_1038_s41592_019_0498_4
- Format
- –
- Schlagworte
- HeLa Cells, Humans, Insulin Receptor Substrate Proteins - metabolism, Isomerism, MCF-7 Cells, Phosphatidylinositol 3-Kinases - metabolism, Phosphopeptides - analysis, Phosphoproteins - analysis, Phosphorylation, Proteome - analysis, Proto-Oncogene Proteins c-akt - metabolism, Search Engine - methods, Signal Transduction, Tandem Mass Spectrometry