Details

Autor(en) / Beteiligte

• De Munari, Sergio
• Barassi, Paolo
• Cerri, Alberto
• Fedrizzi, Giorgio
• Gobbini, Mauro
• Mabilia, Massimo
• Melloni, Piero

Titel

A New Approach to the Design of Novel Inhibitors of Na+,K+-ATPase:  17α-Substituted Seco-D 5β-Androstane as Cassaine Analogues

Ist Teil von

• Journal of medicinal chemistry, 1998-07, Vol.41 (16), p.3033-3040

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

1998

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A new three-dimensional model for the relative binding mode of cassaine 1 and digitoxigenin 2 at the digitalis receptor site is proposed on the basis of the structural and conformational similarities among 1, 2 and its 14,15-seco analogues 3 and 4. Accordingly, the speculation that also 17α-substituted derivatives of the digitalis 5β,14β-androstane skeleton could efficiently bind to the Na+,K+-ATPase receptor is put forward and verified through the synthesis of some related compounds. The binding affinity shown by 2-(N,N-dimethylamino)ethyl 3β,14-dihydroxy-5β,14β-androstane-17α-acrylate 6 (IC50 = 5.89 μM) and, much more significantly, by the corresponding 14,15-seco-14-oxo derivative 9 (IC50 = 0.12 μM) substantiates the new hypothesis and opens new prospects to the design of novel inhibitors of Na+,K+-ATPase as potential positive inotropic compounds.