Details

Autor(en) / Beteiligte

• Robert, Marie-Claude
• Oberson, Jean-Marie
• Stadler, Richard H

Titel

Model Studies on the Formation of Monochloropropanediols in the Presence of Lipase

Ist Teil von

• Journal of agricultural and food chemistry, 2004-08, Vol.52 (16), p.5102-5108

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• The formation of chloropropanols was investigated using model systems comprised of lipase, vegetable oil or fat, water, and sodium chloride. The results showed that measurable levels of the foodborne carcinogen 3-chloro-1,2-propanediol (3-MCPD) are formed in the presence of commercially available lipases of mammalian, vegetable, and fungal origins, incubated at temperatures of 40 °C. The highest yield of 3-MCPD was obtained in reaction mixtures containing lipase from Rhizopus oryzae, and all the lipases studied exhibited a high hydrolytic activity toward triglycerides from palm and peanut oil. In contrast, hydrolysis over time and the yield of 3-MCPD in olive and sunflower oils were significantly lower (up to 10-fold), possibly linked to the relatively lower amount (<18%) of saturated fatty acids in these oils. We provide here for the first time evidence that lipases are able to induce the formation of chloropropanols under model system conditions. However, the key intermediates and precise mechanistic aspects governing the formation of 3-MCPD in the presence of lipase still need to be elucidated. Keywords: Chloropropanols; chlorohydrins; 3-MCPD; 3-chloro-1,2-propanediol; formation; analysis; food; process contaminant; lipase