Details

Autor(en) / Beteiligte

• Keinänen, Markku
• Oldham, Neil J
• Baldwin, Ian T

Titel

Rapid HPLC Screening of Jasmonate-Induced Increases in Tobacco Alkaloids, Phenolics, and Diterpene Glycosides in Nicotiana attenuata

Ist Teil von

• Journal of agricultural and food chemistry, 2001-08, Vol.49 (8), p.3553-3558

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• A rapid, HPLC-based screening procedure for the main classes of secondary metabolites in Nicotiana attenuata leaves (alkaloids, phenolics, and diterpene glycosides) is reported. In a single step, leaves are extracted in aqueous acidified (0.5% acetic acid) methanol, and the extracted compounds are separated by reversed-phase HPLC with an acidic water/acetonitrile gradient in <30 min. The utility of the method in quantifying changes in the secondary metabolites after methyl jasmonate treatment of the plants, a treatment known to elicit resistance to herbivores in nature, is illustrated. Methyl jasmonate treatment elicited dramatic increases in some secondary metabolites (caffeoylputrescine, nicotine, and diterpene glycosides increased 12.5-, 1.4-, and 1.9-fold, respectively) but left others, such as rutin, unchanged. Such broad-based analytical screens will help characterize environmental and genetic changes in secondary metabolite profiles. Keywords: Nicotiana; tobacco; tobacco alkaloids; nicotine; phenolics; conjugated polyamines; diterpene glycosides; liquid chromatography; mass spectrometry; jasmonic acid