Details

Autor(en) / Beteiligte

• Chen, Qian
• Xie, Baogang
• Zhou, Liping
• Sun, Lili
• Li, Shanshan
• Chen, Yuhan
• Shi, Shan
• Li, Yang
• Yu, Mingan
• Li, Wei

Titel

A Tailor-Made Self-Sufficient Whole-Cell Biocatalyst Enables Scalable Enantioselective Synthesis of (R)‑3-Quinuclidinol in a High Space-Time Yield

Ist Teil von

• Organic process research & development, 2019-09, Vol.23 (9), p.1813-1821

Ort / Verlag

American Chemical Society

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A self-sufficient two-in-one whole-cell biocatalyst combining ketoreductase and cofactor regenerating enzyme activities has been developed and successfully utilized to synthesize (R)-3-quinuclidinol with an increase in the reaction rate of 3-fold over the native enzymes at low biocatalysts loading (∼1.65%), excellent enantioselectivity, impressive substrate concentration of 486 g L–1, and high space-time yield of (R)-3-quinuclidinol up to 1505.5 g L–1 d–1, which was the highest ever reported. These results demonstrated that the newly developed self-sufficient biocatalyst could be useful for synthetic and industrial application in synthesis of (R)-3-quinuclidinol, essential for the production of solifenacin, revatropate, and aclidinium, with better clinical outcome than those currently available.