Details

Autor(en) / Beteiligte

• Foster, Jeffrey C
• Radzinski, Scott C
• Zou, Xianlin
• Finkielstein, Carla V
• Matson, John B

Titel

H 2 S-Releasing Polymer Micelles for Studying Selective Cell Toxicity

Ist Teil von

• Molecular pharmaceutics, 2017-04, Vol.14 (4), p.1300-1306

Ort / Verlag

United States

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• We report the preparation of S-aroylthiooxime (SATO) functionalized amphiphilic block copolymer micelles that release hydrogen sulfide (H S), a gaseous signaling molecule of relevance to various physiological and pathological conditions. The micelles release H S in response to cysteine with a half-life of 3.3 h, which is substantially slower than a related small molecule SATO. Exogenous administration of H S impacts growth and proliferation of cancer cells; however, the limited control over H S generation from inorganic sulfide sources results in conflicting reports. Therefore, we compare the cellular cytotoxicity of SATO-functionalized micelles, which release H S in a sustained manner, to Na S, which releases H S in a single dose. Our results show that H S-releasing micelles significantly reduce the survival of HCT116 colon cancer cells relative to Na S, GYY4137, and a small molecule SATO, indicating that release kinetics may play an important role in determining toxicity of H S toward cancer cells. Furthermore, H S-releasing micelles are well tolerated by immortalized fibroblasts (NIH/3T3 cells), suggesting a selective toxicity of H S toward cancer cells.