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•A new protocol for immobilization of lipase CaLB on Biosilified Pd nanoparticles.•DKR of α-methylbenzylamine in one step with a new hybrid biocatalyst.•Lower protein loads and smaller amounts of Pd were applied.
In this work lipase CaLB was immobilized on functionalized Pd-SiO2 nanoparticles in order to simplify the DKR of α-methylbenzylamine. Hybrid biocatalysts showed immobilization efficiencies of 82%, 80% and 76% when containing 1, 5 and 10% of Pd respectively. On DKR reaction values of ee > 99% and conversion of 82% were found with only 1% of Pd, generating a productivity of 2.21 mg of product h−1 mg of support−1 against 0.76 found by N435®. Compared to commercial N435®, the novel biocatalysts showed protein loads about 15-fold lower and higher activity, demonstrating competitive performances and high industrial applications.