Details

Autor(en) / Beteiligte

• de Souza, Stefânia P.
• Leão, Raquel A.C.
• Bassut, Jonathan F.
• Leal, Ivana C.R.
• Wang, Shuai
• Ding, Qiqi
• Li, Yingying
• Lam, Frank Leung-Yuk
• de Souza, Rodrigo O.M.A.
• Itabaiana Jr, Ivaldo

Titel

New Biosilified Pd-lipase hybrid biocatalysts for dynamic resolution of amines

Ist Teil von

• Tetrahedron letters, 2017-12, Vol.58 (52), p.4849-4854

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2017

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• [Display omitted] •A new protocol for immobilization of lipase CaLB on Biosilified Pd nanoparticles.•DKR of α-methylbenzylamine in one step with a new hybrid biocatalyst.•Lower protein loads and smaller amounts of Pd were applied. In this work lipase CaLB was immobilized on functionalized Pd-SiO2 nanoparticles in order to simplify the DKR of α-methylbenzylamine. Hybrid biocatalysts showed immobilization efficiencies of 82%, 80% and 76% when containing 1, 5 and 10% of Pd respectively. On DKR reaction values of ee > 99% and conversion of 82% were found with only 1% of Pd, generating a productivity of 2.21 mg of product h−1 mg of support−1 against 0.76 found by N435®. Compared to commercial N435®, the novel biocatalysts showed protein loads about 15-fold lower and higher activity, demonstrating competitive performances and high industrial applications.