Details

Autor(en) / Beteiligte

• Zhu, Hanliang
• Liu, Xiaocheng
• Wang, Yingqi
• Sun, Antao
• Teplý, Tomas
• Korabečná, Marie
• Zhang, Haoqing
• Neuzil, Pavel

Titel

Smallest dual-color qPCR device

Ist Teil von

• Sensors and actuators. B, Chemical, 2023-11, Vol.394, p.134299, Article 134299

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2023

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)- and TxRed-labeled probes. The device measures (110 × 83 × 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection. •Our device is the smallest dual-color qPCR detector to date•The device is capable of processing four samples a time, each with two color PCR multiplexing•We demonstrated the device to perform detection gene E and N from SARS-CoV-2•The device can also perform single color measurement using intercalating dye as well as RT-LAMP