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A fluorescence sensing platform with the MnO2 nanosheets as an effective oxidant for glutathione detection
Ist Teil von
Sensors and actuators. B, Chemical, 2017-11, Vol.252, p.30-36
Ort / Verlag
Elsevier B.V
Erscheinungsjahr
2017
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
[Display omitted]
•A sensitive, selective, rapid and cost-effective method has been developed for fluorescence sensing of GSH.•The MnO2 nanosheets served as both oxidant and recognition element.•This platform can be used for detecting the GSH in cell extracts.
Herein, a sensitive, selective, rapid and cost-effective fluorescence sensing platform has been designed for the detection of glutathione (GSH). In this platform, the manganese dioxide (MnO2) nanosheets served as both oxidant, which could effectively oxidize o-phenylenediamine (OPDA) to produce fluorescence for highly sensitive detection, and recognition element. When GSH was introduced, the MnO2 nanosheets were reduced to Mn2+, which inhibited the formation of fluorescent oxidation products of OPDA. Under optimal conditions, the MnO2-OPDA sensing platform showed very sensitive response to GSH in the range of 10nM–40μM. The detection limit of GSH reached as low as 10nM. The high selectivity of inhibition of fluorescent product formation by GSH allows this platform to be used for detecting GSH in practical biological samples (such as cell extracts).